Alpha MED64 MED-A64HE1 User's Guide download pdf (Page 49)

Mobius Tutorial: Cahper 3 - Spike Sorter 43

3-1. Recording spontaneous neuronal spikes (single unit)

Chapter 3 Spike Sorter

This chapter will teach you how to record and analyze spontaneous neuronal spikes (single unit activi-

ties) either from neuron cultures or brain slices using available workflow templates. In this chapter you

will learn:

1. How to record spontaneous neuronal spikes (single unit activities)

2. How to test drug effects

3. How to replay and analyze spontaneous neuron spikes (single unit activities)

3-1. Recording spontaneous neuronal spikes (single unit)

There are 5 workflow templates available for recording spontaneous neuronal spikes (single unit):

1. Spontaneous_recording (Recording WITHOUT any real-time analysis)

2. Spike_recording (Recording with extracting spikes and their frequency analysis)

3. Spike_recording_filter (2 + filter)

4. Spike_recording_cluster (Recording with extracting, clustering spikes, and their frequency analyses)

5. Spike_recording_filter_cluster (4+filter)

Opening the workflow template

1. Click [Workflow] > [New] > [From Template]. (Fig.3-1.1)

2. Select either of [64MD1_1280x1024] or [64MD1_1920x1080] folder, depending on the size of your

display monitor. Select [Spikes] > [Spontaneous_recording] folders. Select the workflow template.

• Go to [Basic_recording] folder for the “Spontaneous_recording” workflow template.

NOTICE:

Connect your PC to the

MED64 amplifiers and turn

on both amplifiers to open

recording workflows.

Fig.3-1.1.

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Alpha MED64 MED-A64HE1 User's Guide Page 49